TAKAITACCE DA BAYANIN GWAJI
Visceral leishmaniasis, ko Kala-azar, cuta ce da ke yaduwa ta hanyar nau'ikan nau'ikan L. donovani da yawa.Hukumar Lafiya ta Duniya (WHO) ta yi kiyasin cewa cutar za ta shafi kusan mutane miliyan 12 a kasashe 88.Ana yada shi ga mutane ta hanyar cizon yashi na Phlebotomus, wanda ke kamuwa da kamuwa da cutar ta hanyar ciyar da dabbobi masu kamuwa da cuta.Ko da yake cuta ce da ake samu a ƙasashe matalauta, a Kudancin Turai, ta zama kan gaba wajen kamuwa da cutar kanjamau.Gano kwayoyin L. donovani daga jini, kasusuwan kasusuwa, hanta, lymph nodes ko splin yana ba da ma'anar ganewar asali.Binciken serological na anti-L.Donovani IgM an samo shi azaman kyakkyawan alama ga m Visceral leishmaniasis.Gwaje-gwajen da aka yi amfani da su a asibiti sun haɗa da ELISA, antibody fluorescent ko gwajin agglutination kai tsaye 4-5.Kwanan nan, yin amfani da ƙayyadaddun furotin na L. donovani a cikin gwajin ya inganta hankali da ƙayyadaddun abu sosai.
Gwajin gaggawa na Leishmania IgG/IgM Combo Rapid Test shine sake haɗe sunadaran gina jiki gwajin serological, wanda ke gano IgG da IgM rigakafi ga L. Donovani lokaci guda.Gwajin yana ba da ingantaccen sakamako a cikin mintuna 15 ba tare da wani kayan aiki ba.
KA'IDA
Gwajin gaggawa na Leishmania IgG/IgM shine gwajin rigakafi na chromatographic na gefe.Kaset ɗin gwajin ya ƙunshi: 1) wani kushin conjugate mai launin burgundy mai ɗauke da recombinant L. donovani antigen da aka haɗa da colloid zinariya (Leishmania conjugates) da zomo IgG-gold conjugates, 2) wani nitrocellulose membrane tsiri dauke da biyu gwajin makada (T1 da T2 bands). da kuma bandeji mai sarrafawa (C band).An riga an riga an rufe rukunin T1 tare da IgM anti-mutane na monoclonal don gano anti-L.Donovani IgM, T2 band an precoated tare da reagents don gano anti-L.donovani IgG, kuma rukunin C an riga an rufe shi da goat anti zomo IgG.
Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.L. donovani IgM idan akwai a cikin samfurin zai ɗaure ga haɗin gwiwar Leishmania.An kama immunocomplex a kan membrane ta hanyar riga-kafi na IgM anti-an adam antibody, samar da wani burgundy launi T1 band, yana nuna L. donovani IgM tabbatacce sakamakon gwajin.L. donovani IgG idan akwai a cikin samfurin zai ɗaure ga haɗin gwiwar Leishmania.Immunocomplex an kama shi ta hanyar reagents da aka riga aka rufawa akan membrane, suna samar da rukunin T2 mai launin burgundy, yana nuna sakamako mai kyau na L. donovani IgG.
Rashin kowane nau'in T (T1 da T2) yana nuna sakamako mara kyau.Gwajin yana ƙunshe da iko na ciki (C band) wanda yakamata ya nuna band mai launin burgundy na immunocomplex na goat anti rabbit IgG/ rabbit IgG-gold conjugate ba tare da la’akari da ci gaban launi akan kowane rukunin T ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
