TAKAITACCE DA BAYANIN GWAJI
Leptospirosis na faruwa a duk duniya kuma matsala ce mai sauƙi zuwa mai tsanani ga mutane da dabbobi, musamman a wuraren da ke da yanayi mai zafi da danshi.Tafkunan halitta na leptospirosis rodents ne da kuma nau'ikan dabbobi masu shayarwa na gida.Cutar da ɗan adam ta haifar da L. interrogans, memba na pathogenic na jinsin Leptospira.Cutar na yaduwa ta hanyar fitsari daga dabbar da ta kwana.
Bayan kamuwa da cuta, leptospires suna cikin jini har sai an share su bayan kwanaki 4 zuwa 7 bayan samar da maganin L.interrogans antibodies, farkon na IgM class.Al'adar jini, fitsari da ruwan cerebrospinal wata hanya ce mai tasiri don tabbatar da ganewar asali a cikin makonni 1 zuwa 2 bayan bayyanar.Binciken serological na antiL.interrogans antibodies kuma hanya ce ta bincike ta gama gari.Ana samun gwaje-gwaje a ƙarƙashin wannan rukuni: 1) Gwajin agglutination microscopic (MAT);2) ELISA;3) Gwajin antibody fluorescent kaikaice (IFATs).Koyaya, duk hanyoyin da aka ambata a sama suna buƙatar ƙayyadaddun kayan aiki da ƙwararrun ƙwararrun masana.
Leptospira IgG/IgM gwaji ne mai sauƙi na serological wanda ke amfani da antigens daga L. interrogans kuma yana gano ƙwayoyin IgG da IgM ga waɗannan ƙwayoyin cuta a lokaci guda.Za a iya yin gwajin ta ƙwararrun ma'aikatan da ba su da horo ko kuma ƙwararrun ma'aikata, ba tare da ƙaƙƙarfan kayan aikin dakin gwaje-gwaje ba kuma ana samun sakamakon a cikin mintuna 15.
KA'IDA
Kit ɗin gwajin gaggawa na Leptospira IgG/IgM shine chromatographic kwarara na gefe
immunoassay.Kaset ɗin gwajin ya ƙunshi: 1) kushin conjugate mai launin burgundy mai ɗauke da recombinant L. interrogans antigens conjugated da colloid zinariya (Leptospira conjugates) da zomo IgG-gold conjugates, 2) wani nitrocellulose membrane tsiri dauke da biyu gwajin makada (M da G makada). da kuma bandeji mai sarrafawa (C band).M band an riga an rufe shi da monoclonal anti-human IgM don gano anti-L.. interrogans IgM, G band an riga an rufe shi da reagents don gano anti-L.interrogans IgG, kuma rukunin C an riga an yi masa rufi da goat anti zomo IgG.
Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.IgM anti-L.. masu tambayoyi idan akwai a cikin samfurin za su ɗaure ga haɗin gwiwar Leptospira.Immunocomplex an kama shi a jikin membrane ta riga-kafin rigakafin IgM na ɗan adam wanda aka riga aka rufa masa, yana samar da band ɗin M mai launin burgundy, yana nuna L.. interrogans IgM tabbatacce sakamakon gwajin.IgG anti-L.. masu tambayoyi idan akwai a cikin samfurin za su ɗaure ga haɗin gwiwar Leptospira.Immunocomplex an kama shi ta hanyar reagents da aka riga aka rufawa akan membrane, suna samar da rukunin G mai launin burgundy, yana nuna L.. interrogans IgG tabbatacce sakamakon gwajin.
Rashin kowane makada na gwaji (M da G) yana nuna sakamako mara kyau.Gwajin yana ƙunshe da iko na ciki (C band) wanda yakamata ya nuna band mai launin burgundy na immunocomplex na goat anti rabbit IgG/ rabbit IgG-gold conjugate ba tare da la’akari da ci gaban launi akan kowane rukunin gwajin ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
