TAKAITACCE DA BAYANIN GWAJI
Tuberculosis cuta ce ta yau da kullun, cuta mai yaɗuwa daga M. TB hominis (Koch's bacillus), lokaci-lokaci ta hanyar M. TB bovis.Huhu sune manufa ta farko, amma kowace gaɓa na iya kamuwa da cuta.
Hadarin kamuwa da tarin fuka ya ragu sosai a karni na 20.Koyaya, bullowar nau'ikan nau'ikan jure magunguna1, musamman a tsakanin majinyata da ke ɗauke da AIDS2, ya sake farfado da sha'awar tarin fuka.An ba da rahoton bullar cutar kusan miliyan 8 a kowace shekara tare da adadin mutuwar miliyan 3 a kowace shekara.Yawan mace-mace ya zarce kashi 50 cikin 100 a wasu kasashen Afirka da ke fama da cutar kanjamau.
Zato na farko na asibiti da binciken binciken rediyo, tare da tabbacin dakin gwaje-gwaje na gaba ta hanyar gwajin sputum da al'adu sune hanyoyin gargajiya (s) a cikin ganewar asali na TB5,6 mai aiki.Duk da haka, waɗannan hanyoyin ko dai ba su da hankali ko kuma suna cin lokaci, musamman ba su dace da marasa lafiya waɗanda ba su iya samar da isasshen sputum, smear-negative, ko kuma ake zargin suna da tarin fuka.
An haɓaka gwajin gaggawa na TB IgG/IgM don rage waɗannan cikas.Gwajin yana gano IgM da IgG anti-M.TB a cikin jini, plasm, ko duka jini a cikin mintuna 15.Wani sakamako mai kyau na IgM yana nuna sabon kamuwa da cuta na M.TB, yayin da amsa mai kyau ta IgG yana nuna kamuwa da cuta ta baya ko na yau da kullun.Yin amfani da takamaiman antigens na M.TB, yana kuma gano IgM anti-M.TB a cikin marasa lafiya da aka yi wa alurar riga kafi tare da BCG.Bugu da kari, gwajin na iya zama
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KA'IDA
Gwajin gaggawa na TB IgG/IgM shine gwajin rigakafi na chromatographic na gefe.Kaset ɗin gwajin ya ƙunshi: 1) wani kushin conjugate mai launin burgundy mai ɗauke da antigens M.TB wanda aka haɗa tare da gwal ɗin colloid (M.TB conjugates) da zomo IgG-gold conjugates, 2) wani tsiri na nitrocellulose mai ɗauke da bandejin gwaji guda biyu (M da G bands. ) da kuma ƙungiyar kulawa (C band).M band an riga an rufe shi da monoclonal anti-human IgM don gano IgM anti-M.TB, rukunin G an riga an rufe shi da reagents don gano IgG anti-M.TB, kuma ƙungiyar C ta riga ta kasance. -mai rufi da goat anti-zomo IgG.
Lokacin da aka ba da isasshen adadin samfurin gwaji a cikin rijiyar samfurin kaset, samfurin yana ƙaura ta hanyar aikin capillary a cikin kaset ɗin.IgM anti-M.TB idan akwai a cikin samfurin zai ɗaure ga haɗin M.TB.An kama immunocomplex akan membrane ta hanyar riga-kafi na anti-an adam IgM antibody, yana samar da band M mai launin burgundy, yana nuna sakamakon M.TB IgM tabbatacce.IgG anti-M.TB, idan akwai a cikin samfurin, zai ɗaure ga haɗin M.TB.Immunocomplex an kama shi ta hanyar reagents da aka riga aka rufawa akan membrane, suna samar da rukunin G mai launin burgundy, yana nuna kyakkyawan sakamakon M.TB IgG.Rashin kowane makada na gwaji (M da G) yana nuna sakamako mara kyau.Gwajin yana ƙunshe da iko na ciki (C band) wanda yakamata ya nuna band mai launin burgundy na immunocomplex na goat anti rabbit IgG/ rabbit IgG-gold conjugate ba tare da la’akari da ci gaban launi akan kowane rukunin T ba.In ba haka ba, sakamakon gwajin ba shi da inganci kuma dole ne a sake gwada samfurin da wata na'ura.
